PCR Based Detection of Cutaneous Leishmaniasis

Background: The second most common illness after malaria that is transmitted by vectors and may be lethal if untreated leishmaniasis. Leishmaniasis caused parasites of the genus Leishmania, which are classified into two subgenera, Viannia Leishmania. Methodology: A total 100 sample collected from presumed clinically as CL patients dermatologist, with 50 positive cases negative on Geimsa stain microscopy for Leishman Donovan (LD) bodies. After permission Ethical board in Khyber medical university KPK inform consent patient or their guardian case children was taken. lesion its surroundings were cleaned 70% ethanol, fluid oozing out first prick obtained disperse over a glass slide, left to dry fixed methanol, stained Giemsa stain. Two pricks administered sterile BD syringe at active margin lesion. prick's kept an EDTA tube PCR usage -20°C IBMS laboratory KMU Peshawar. Results: Out patients, 53 female 47 male. age ranged year 89 year. 64 36 CL. microscopic 37cases PCR. 27 (54%) statistically significant difference found between method detection Conclusion: finding our study specifies base analysis superior diagnosis Therefore, we recommend use along staining order improve quality sensitivity routine disease. Keywords: Polymerase Chain Reaction, staining, Cutaneous leishmaniasis